Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Lingfeng Yu; Samarendra Mohanty; Gangjun Liu; Suzanne Genc; Myung K. Kim; Zhongping Chen; Michael W. Berns

doi:10.1117/12.809761

12 February 2009 Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Myung K. Kim, Zhongping Chen, Michael W. Berns

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 718210 (2009) https://doi.org/10.1117/12.809761
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system, which takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intra-cellular structures, being exposed to the laser microbeam, which was not possible using conventional phase-contrast microscopy.

Citation Download Citation

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Myung K. Kim, Zhongping Chen, and Michael W. Berns "Quantitative phase evaluation of dynamic changes on the cell membrane during laser microsurgery", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718210 (12 February 2009); https://doi.org/10.1117/12.809761

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