Laser rastering flow cytometry: fast cell counting and identification

G. Vacca; M. R. Junnarkar; N. R. Goldblatt; M. W. Yee; B. M. Van Slyke; T. C. Briese

doi:10.1117/12.820799

12 February 2009 Laser rastering flow cytometry: fast cell counting and identification

G. Vacca, M. R. Junnarkar, N. R. Goldblatt, M. W. Yee, B. M. Van Slyke, T. C. Briese

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71821T (2009) https://doi.org/10.1117/12.820799
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We describe the concept of laser rastering flow cytometry, where a rapidly scanning laser beam allows counting and classification of cells at much higher rates than currently possible. Modifications to existing flow cytometers to implement the concept include an acousto-optic deflector, fast analog-to-digital conversion, and a two-step digital-signal-processing scheme that handles the high data rates and provides key assay information. Results are shown that prove the concept, demonstrating the ability to resolve closely spaced cells and to measure cells at rates more than an order of magnitude faster than on conventional flow-cytometer-based hematology analyzers.

Citation Download Citation

G. Vacca, M. R. Junnarkar, N. R. Goldblatt, M. W. Yee, B. M. Van Slyke, and T. C. Briese "Laser rastering flow cytometry: fast cell counting and identification", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821T (12 February 2009); https://doi.org/10.1117/12.820799

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