Multi-focus CARS microscopy using microlens array scanner for realtime molecular spectral imaging

Mamoru Hashimoto; Takeo Minamikawa; Tsutomu Araki; Katsumasa Fujita; Satoshi Kawata

doi:10.1117/12.808699

25 February 2009 Multi-focus CARS microscopy using microlens array scanner for realtime molecular spectral imaging

Mamoru Hashimoto, Takeo Minamikawa, Tsutomu Araki, Katsumasa Fujita, Satoshi Kawata

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71830R (2009) https://doi.org/10.1117/12.808699
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We realized realtime molecular imaging with low excitation laser intensity using a multi-focus excitation CARS (coherent anti-Stokes Raman scattering) microscope. We demonstrated realtime CARS images of polystyrene beads and lipid vesicles. Time series CARS images of the polystyrene beads in water was obtained with the frame rate of 30 fps. The three dimensional lipids vesicle which consists of 50 slices was observed within 7 s (100 ms/image).

Citation Download Citation

Mamoru Hashimoto, Takeo Minamikawa, Tsutomu Araki, Katsumasa Fujita, and Satoshi Kawata "Multi-focus CARS microscopy using microlens array scanner for realtime molecular spectral imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830R (25 February 2009); https://doi.org/10.1117/12.808699

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