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13 February 2009 Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation
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Abstract
High-order dispersion of ultrashort laser pulses (with ~100 nm bandwidth) is shown to account for significant reduction of two-photon excitation fluorescence and second harmonic generation signal produced at the focal plane of a laser-scanning two-photon microscope. The second- and third-order corrections recover 20-40% of the signal intensity expected for a transform-limited laser pulse, while the rest depends on the proper compensation of higher-order terms. It can be accomplished through the use of a pulse shaper by measuring and correcting all nonlinear spectral phase distortions.
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Yair Andegeko, Dmitry Pestov, Vadim V. Lozovoy, and Marcos Dantus "Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830W (13 February 2009); doi: 10.1117/12.808429; https://doi.org/10.1117/12.808429
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