13 February 2009 Adaptive optics for multiphoton microscopy
Author Affiliations +
Abstract
Specimen-induced aberrations are frequently encountered in high resolution microscopy, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations distort the focal spot causing a reduction in resolution and, often more importantly, reduced signal level and contrast. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have developed an adaptive two-photon fluorescence microscope to correct for these aberrations. Unlike a conventional adaptive optics system, the microscope does not include a wavefront sensor but uses an efficient sensorless optimisation scheme to obtain optimum aberration correction.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Delphine Débarre, Tony Wilson, Martin J. Booth, "Adaptive optics for multiphoton microscopy", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831J (13 February 2009); doi: 10.1117/12.810367; https://doi.org/10.1117/12.810367
PROCEEDINGS
8 PAGES


SHARE
Back to Top