13 February 2009 Adaptive optics for multiphoton microscopy
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Abstract
Specimen-induced aberrations are frequently encountered in high resolution microscopy, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations distort the focal spot causing a reduction in resolution and, often more importantly, reduced signal level and contrast. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have developed an adaptive two-photon fluorescence microscope to correct for these aberrations. Unlike a conventional adaptive optics system, the microscope does not include a wavefront sensor but uses an efficient sensorless optimisation scheme to obtain optimum aberration correction.
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Delphine Débarre, Delphine Débarre, Tony Wilson, Tony Wilson, Martin J. Booth, Martin J. Booth, } "Adaptive optics for multiphoton microscopy", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831J (13 February 2009); doi: 10.1117/12.810367; https://doi.org/10.1117/12.810367
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