13 February 2009 Imaging dental sections with polarization-resolved SHG and time-resolved autofluorescence
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In this study, we are using two-photon (2-p) excited autofluorescence and second harmonic (SH) as imaging modalities to investigate dental sections that contains the enamel and the dentin. The use of near-infrared wavelengths for multiphoton excitation greatly facilitates the observation of these sections due to the hard tissue's larger index of refraction and highly scattering nature. Clear imaging can be achieved without feature altering preparation procedures of the samples. Specifically, we perform polarization resolving on SH and lifetime analysis on autofluorescence. Polarization resolved SH reflects the preferred orientation of collagen while very different autofluorescence lifetimes are observed from the dentin and the enamel. The origin of 2-p autofluorescence and SH signals are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils exist only in the dentin and dentinoenamel junctions.
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Jun Huang Chen, Jun Huang Chen, Po-Yen Lin, Po-Yen Lin, Stephen C. Y. Hsu, Stephen C. Y. Hsu, Fu-Jen Kao, Fu-Jen Kao, } "Imaging dental sections with polarization-resolved SHG and time-resolved autofluorescence", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831N (13 February 2009); doi: 10.1117/12.808524; https://doi.org/10.1117/12.808524

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