Coherent control in multiphoton fluorescence imaging

Arijit Kumar De; Debabrata Goswami

doi:10.1117/12.807687

25 February 2009 Coherent control in multiphoton fluorescence imaging

Arijit Kumar De, Debabrata Goswami

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71832B (2009) https://doi.org/10.1117/12.807687
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

In multiphoton fluorescence laser-scanning microscopy ultrafast laser pulses, i.e. light pulses having pulse-width ≤ 1picosecond (1 ps = 10^-12 s), are commonly used to circumvent the low multiphoton absorption cross-sections of common fluorophores. Starting with a discussion on how amplitude modulation of ultrashort pulse-train enhances the two-photon fluorescence providing deep insight into laser-induced photo-thermal damage, the effect of controlling time lag between phase-locked laser pulses on imaging is described. In addition, the prospects of laser pulse-shaping in signal enhancement (by temporal pulse-compression at the sample) and selective excitation of fluorophores (by manipulating the phase and/or amplitude of different frequency components within the pulse) are discussed with promising future applications lying ahead.

Citation Download Citation

Arijit Kumar De and Debabrata Goswami "Coherent control in multiphoton fluorescence imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832B (25 February 2009); https://doi.org/10.1117/12.807687

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available