25 February 2009 Coherent control in multiphoton fluorescence imaging
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In multiphoton fluorescence laser-scanning microscopy ultrafast laser pulses, i.e. light pulses having pulse-width ≤ 1picosecond (1 ps = 10-12 s), are commonly used to circumvent the low multiphoton absorption cross-sections of common fluorophores. Starting with a discussion on how amplitude modulation of ultrashort pulse-train enhances the two-photon fluorescence providing deep insight into laser-induced photo-thermal damage, the effect of controlling time lag between phase-locked laser pulses on imaging is described. In addition, the prospects of laser pulse-shaping in signal enhancement (by temporal pulse-compression at the sample) and selective excitation of fluorophores (by manipulating the phase and/or amplitude of different frequency components within the pulse) are discussed with promising future applications lying ahead.
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Arijit Kumar De, Arijit Kumar De, Debabrata Goswami, Debabrata Goswami, } "Coherent control in multiphoton fluorescence imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832B (25 February 2009); doi: 10.1117/12.807687; https://doi.org/10.1117/12.807687

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