SHIM and TPEM: getting more information from non linear excitation

Paolo Bianchini; Alberto Diaspro

doi:10.1117/12.809248

13 February 2009 SHIM and TPEM: getting more information from non linear excitation

Paolo Bianchini, Alberto Diaspro

Author Affiliations +

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718335 (2009) https://doi.org/10.1117/12.809248
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Several endogenous protein structures give rise to second harmonic generation (SHG) - second order nonabsorbative energy doubling of an excitation laser line. The orientation of collagen fibers within tissues such as tendons or ligaments is of primary importance. In this study, we propose a method to map the orientation of collagen fibers of a tendon. The method uses only few images acquired at specific polarizations of the input laser beam by rotating the sample on the stage. This procedure is implemented both in backward and forward scattering pathways. The improved details should clarify the backscattered signal nature.

Citation Download Citation

Paolo Bianchini and Alberto Diaspro "SHIM and TPEM: getting more information from non linear excitation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718335 (13 February 2009); https://doi.org/10.1117/12.809248

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE