3 March 2009 Live TIRF microscopy at 100nm resolution through structured illumination
Author Affiliations +
Abstract
Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
P. Kner, B. Chhun, E. Griffis, L. Winoto, L. Shao, M. G. L. Gustafsson, "Live TIRF microscopy at 100nm resolution through structured illumination", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 718417 (3 March 2009); doi: 10.1117/12.812351; https://doi.org/10.1117/12.812351
PROCEEDINGS
9 PAGES


SHARE
Back to Top