High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light

D. Ivanov; V. Shcheslavskiy; I. Märki; M. Leutenegger; T. Lasser

doi:10.1117/12.807596

24 February 2009 High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light

D. Ivanov, V. Shcheslavskiy, I. Märki, M. Leutenegger, T. Lasser

Author Affiliations +

Proceedings Volume 7185, Single Molecule Spectroscopy and Imaging II; 71850D (2009) https://doi.org/10.1117/12.807596
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO₄ laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.

Citation Download Citation

D. Ivanov, V. Shcheslavskiy, I. Märki, M. Leutenegger, and T. Lasser "High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850D (24 February 2009); https://doi.org/10.1117/12.807596

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