24 February 2009 SPDM: single molecule superresolution of cellular nanostructures
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Abstract
Novel methods of visible light microscopy have overcome the limits of resolution hitherto thought to be insurmountable. The localization microscopy technique presented here based on the principles of Spectral Precision Distance Microscopy (SPDM) with conventional fluorophores under special physical conditions allows to measure the spatial distribution of single fluorescence labeled molecules in entire cells with macromolecular precision which is comparable to a macromolecular effective optical resolution. Based on detection of single molecules, in a novel combination of SPDM and Spatially Modulated Illumination (SMI) microscopy, a lateral (2D) effective optical resolution of cellular nanostructures around 10 - 20 nm (about 1/50th of the exciting wavelength) and a three dimensional (3D) effective optical resolution in the range of 40 - 50 nm are achieved.
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Rainer Kaufmann, Rainer Kaufmann, Paul Lemmer, Paul Lemmer, Manuel Gunkel, Manuel Gunkel, Yanina Weiland, Yanina Weiland, Patrick Müller, Patrick Müller, Michael Hausmann, Michael Hausmann, David Baddeley, David Baddeley, Roman Amberger, Roman Amberger, Christoph Cremer, Christoph Cremer, } "SPDM: single molecule superresolution of cellular nanostructures", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850J (24 February 2009); doi: 10.1117/12.809109; https://doi.org/10.1117/12.809109
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