Mirror-based broadband scanner with minimized aberration

Jiun-Yann Yu; Yu-Yi Tzeng; Chen-Han Huang; Hsiang-Chen Chui; Shi-Wei Chu

doi:10.1117/12.807634

24 February 2009 Mirror-based broadband scanner with minimized aberration

Jiun-Yann Yu, Yu-Yi Tzeng, Chen-Han Huang, Hsiang-Chen Chui, Shi-Wei Chu

Proceedings Volume 7185, Single Molecule Spectroscopy and Imaging II; 718518 (2009) https://doi.org/10.1117/12.807634
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

To obtain specific biochemical information in optical scanning microscopy, labeling technique is routinely required. Instead of the complex and invasive sample preparation procedures, incorporating spectral acquisition, which commonly requires a broadband light source, provides another mechanism to enhance molecular contrast. But most current optical scanning system is lens-based and thus the spectral bandwidth is limited to several hundred nanometers due to anti-reflection coating and chromatic aberration. The spectral range of interest in biological research covers ultraviolet to infrared. For example, the absorption peak of water falls around 3 μm, while most proteins exhibit absorption in the UV-visible regime. For imaging purpose, the transmission window of skin and cerebral tissues fall around 1300 and 1800 nm, respectively. Therefore, to extend the spectral bandwidth of an optical scanning system from visible to mid-infrared, we propose a system composed of metallic coated mirrors. A common issue in such a mirror-based system is aberrations induced by oblique incidence. We propose to compensate astigmatism by exchanging the sagittal and tangential planes of the converging spherical mirrors in the scanning system. With the aid of an optical design software, we build a diffraction-limited broadband scanning system with wavefront flatness better than λ/4 at focal plane. Combined with a mirror-based objective this microscopic system will exhibit full spectral capability and will be useful in microscopic imaging and therapeutic applications.

Citation Download Citation

Jiun-Yann Yu, Yu-Yi Tzeng, Chen-Han Huang, Hsiang-Chen Chui, and Shi-Wei Chu "Mirror-based broadband scanner with minimized aberration", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 718518 (24 February 2009); https://doi.org/10.1117/12.807634

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KEYWORDS

Monochromatic aberrations

Mirrors

Spherical lenses

Optical scanning systems

Wavefronts

Objectives

Absorption

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