A multi-spectral dark-field reflectance microscope for tumor margin delineation during breast lumpectomy

Venkataramanan Krishnaswamy; Wendy A. Wells; Ashley M. Laughney; Kimberley S. Samkoe; Brian W. Pogue

doi:10.1117/12.809193

24 February 2009 A multi-spectral dark-field reflectance microscope for tumor margin delineation during breast lumpectomy

Venkataramanan Krishnaswamy, Wendy A. Wells, Ashley M. Laughney, Kimberley S. Samkoe, Brian W. Pogue

Author Affiliations +

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 718710 (2009) https://doi.org/10.1117/12.809193
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Infiltrating neoplastic epithelia induce ultra-structure changes in tissue providing an intrinsic contrast in terms of their local light scattering response. Imaging systems that can enhance this contrast allow for better visualization of tumor boundaries and thus have enormous potential in guiding complex surgical procedures like breast lumpectomy. Highly localized reflectance measurement probes can quantify scattering changes in tissues in situ, but in order to be useful in surgical settings these techniques require an extension to imaging. A novel microsampling reflectance imaging system has been developed to allow rapid quantitative imaging of ultra-structure associated scattering changes in tissues in situ. The imaging system is described in terms of its design, construction and testing for multi-wavelength, telecentric, darkfield illumination and confocal spectroscopic detection, with imaging fields of up to 1.5 cm × 1.5 cm at 100 microns resolution. Spatial confinement of the incident and detected light allows for direct sampling of the scattering spectrum in tissues in situ and the telecentric design ensures consistent sampling of the scattering phase function throughout the entire imaging field. The imaging system was modeled and optimized using the ZEMAX optical design software. Description of the design and results from the optimization process are presented.

Citation Download Citation

Venkataramanan Krishnaswamy, Wendy A. Wells, Ashley M. Laughney, Kimberley S. Samkoe, and Brian W. Pogue "A multi-spectral dark-field reflectance microscope for tumor margin delineation during breast lumpectomy", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 718710 (24 February 2009); https://doi.org/10.1117/12.809193

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available