Increased in vivo skin penetration of quantum dots with UVR and in vitro quantum dot cytotoxicity

Luke Mortensen; Hong Zheng; Renea Faulknor; Anna De Benedetto; Lisa Beck; Lisa A. DeLouise

doi:10.1117/12.809215

3 March 2009 Increased in vivo skin penetration of quantum dots with UVR and in vitro quantum dot cytotoxicity

Luke Mortensen, Hong Zheng, Renea Faulknor, Anna De Benedetto, Lisa Beck, Lisa A. DeLouise

Proceedings Volume 7189, Colloidal Quantum Dots for Biomedical Applications IV; 718919 (2009) https://doi.org/10.1117/12.809215
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The growing presence of quantum dots (QD) in a variety of biological, medical, and electronics applications means an increased risk of human exposure in manufacturing, research, and consumer use. However, very few studies have investigated the susceptibility of skin to penetration of QD - the most common exposure route- and the results of those that exist are conflicting. This suggests that a technique allowing determination of skin barrier status and prediction of skin permeability to QD would be of crucial interest as recent findings have provided evidence of in vitro cytotoxicity and long-term in vivo retention in the body for most QD surface chemistries. Our research focuses on barrier status of the skin (intact and with ultraviolet radiation induced barrier defect) and its impact on QD skin penetration. These model studies are particularly relevant to the common application condition of NP containing sunscreen and SPF cosmetics to UV exposed skin. Herein we present our initial efforts to develop an in vivo model of nanoparticle skin penetration using the SKH-1 hairless mouse with transepidermal water loss (TEWL) to evaluate skin barrier status and determine its ability to predict QD penetration. Our results show that ultraviolet radiation increases both TEWL and skin penetration of QD. Additionally, we demonstrate cytotoxic potential of QD to skin cells using a metastatic melanoma cell line. Our research suggests future work in specific targeting of nanoparticles, to prevent or enhance penetration. This knowledge will be used to develop powerful therapeutic agents, decreased penetration cosmetic nanoparticles, and precise skin cancer imaging modalities.

Citation Download Citation

Luke Mortensen, Hong Zheng, Renea Faulknor, Anna De Benedetto, Lisa Beck, and Lisa A. DeLouise "Increased in vivo skin penetration of quantum dots with UVR and in vitro quantum dot cytotoxicity", Proc. SPIE 7189, Colloidal Quantum Dots for Biomedical Applications IV, 718919 (3 March 2009); https://doi.org/10.1117/12.809215

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