Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney; Gregg B. Fields; Samuel Achilefu; W. Barry Edwards

doi:10.1117/12.810096

20 February 2009 Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney, Gregg B. Fields, Samuel Achilefu, W. Barry Edwards

Proceedings Volume 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications; 719011 (2009) https://doi.org/10.1117/12.810096
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ..-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

Citation Download Citation

Philip P. Cheney, Gregg B. Fields, Samuel Achilefu, and W. Barry Edwards "Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging", Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 719011 (20 February 2009); https://doi.org/10.1117/12.810096

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