Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice

Yoshinori Harada; Hirokazu Furuta; Yasutoshi Murayama; Ping Dai; Yuta Fujikawa; Yasuteru Urano; Tetsuo Nagano; Koki Morishita; Akira Hasegawa; Tetsuro Takamatsu

doi:10.1117/12.809063

20 February 2009 Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice

Yoshinori Harada, Hirokazu Furuta, Yasutoshi Murayama, Ping Dai, Yuta Fujikawa, Yasuteru Urano, Tetsuo Nagano, Koki Morishita, Akira Hasegawa, Tetsuro Takamatsu

Author Affiliations +

Proceedings Volume 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications; 719019 (2009) https://doi.org/10.1117/12.809063
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Tumor invasion to the peritoneum is a poor prognostic factor in cancer patients. Accurate diagnosis of disseminated peritoneal tumors is essential to accurate cancer staging. To date, peritoneal washing cytology during laparotomy has been used for diagnosis of peritoneal dissemination of gastrointestinal cancer, but its sensitivity has not been satisfactory. Thus, a more direct approach is indispensable to detect peritoneal dissemination in vivo. Fluorescein diacrylate (FDAcr) is an esterase-sensitive fluorescent probe derived from fluorescein. In cancer cells, fluorescent fluorescein generated by exogenous application of FDAcr selectively deposits owing to its stronger hydrolytic enzyme activity and its lower leakage rate. We examined whether FDAcr can specifically detect disseminated peritoneal tumors in athymic nude mouse models. Intraperitoneally administered FDAcr revealed disseminated peritoneal microscopic tumors not readily recognized on white-light imaging. These results suggest that FDAcr is a useful probe for detecting disseminated peritoneal tumors.

Citation Download Citation

Yoshinori Harada, Hirokazu Furuta, Yasutoshi Murayama, Ping Dai, Yuta Fujikawa, Yasuteru Urano, Tetsuo Nagano, Koki Morishita, Akira Hasegawa, and Tetsuro Takamatsu "Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice", Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 719019 (20 February 2009); https://doi.org/10.1117/12.809063

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