20 February 2009 Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice
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Tumor invasion to the peritoneum is a poor prognostic factor in cancer patients. Accurate diagnosis of disseminated peritoneal tumors is essential to accurate cancer staging. To date, peritoneal washing cytology during laparotomy has been used for diagnosis of peritoneal dissemination of gastrointestinal cancer, but its sensitivity has not been satisfactory. Thus, a more direct approach is indispensable to detect peritoneal dissemination in vivo. Fluorescein diacrylate (FDAcr) is an esterase-sensitive fluorescent probe derived from fluorescein. In cancer cells, fluorescent fluorescein generated by exogenous application of FDAcr selectively deposits owing to its stronger hydrolytic enzyme activity and its lower leakage rate. We examined whether FDAcr can specifically detect disseminated peritoneal tumors in athymic nude mouse models. Intraperitoneally administered FDAcr revealed disseminated peritoneal microscopic tumors not readily recognized on white-light imaging. These results suggest that FDAcr is a useful probe for detecting disseminated peritoneal tumors.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yoshinori Harada, Yoshinori Harada, Hirokazu Furuta, Hirokazu Furuta, Yasutoshi Murayama, Yasutoshi Murayama, Ping Dai, Ping Dai, Yuta Fujikawa, Yuta Fujikawa, Yasuteru Urano, Yasuteru Urano, Tetsuo Nagano, Tetsuo Nagano, Koki Morishita, Koki Morishita, Akira Hasegawa, Akira Hasegawa, Tetsuro Takamatsu, Tetsuro Takamatsu, } "Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice", Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 719019 (20 February 2009); doi: 10.1117/12.809063; https://doi.org/10.1117/12.809063

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