27 February 2009 Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification
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Abstract
We report a system for optical trapping, manipulation and imaging of live cells in close to real-time with a wide field of view. A fs-pulsed Ti:Sapphire source was used to trap cells whilst an Ar+ laser was used for confocal imaging. A single 10x/0.4NA lens and a dual scanning system enabled independent trapping, imaging and movement of cells over a field of view >1mm2. We will present movies of controlled T cell and dendritic cell movement and interaction. This enables the functional study of cell-signalling processes that are fundamental to understanding the behaviour of the immune system and beyond.
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J. Harris, J. Harris, G. McConnell, G. McConnell, } "Wide horizons for optical trapping: simultaneous optical manipulation and confocal imaging of live T cells at low magnification", Proc. SPIE 7193, Solid State Lasers XVIII: Technology and Devices, 719327 (27 February 2009); doi: 10.1117/12.811160; https://doi.org/10.1117/12.811160
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