13 February 2009 New developments in two-photon analysis of human skin in vivo
Author Affiliations +
Abstract
Two-photon imaging of human skin using ultra short laser pulses can be used to obtain information about the state of cells and tissues by means of their natural autofluorescence. Using this method, it is possible to determine whether the normal cell pattern is disturbed or the autofluorescence is influenced by internal or external stimuli. Two-photon fluorescence lifetime imaging (FLIM) can further enhance this providing information about physiological processes, fluorophores (like NAD(P)H, collagen, keratin, elastin, flavins, melanin,...) and external applied probes inside cells and tissue parts. For example the part of the cells metabolism and energy level can be determined by analyzing the NADH regarding its free / bound state and its oxidized / reduced state. The combination of two-photon imaging with FLIM may lead to a better understanding and diagnosis of skin reactions and disorders. We also present some results of in vivo simultaneous collagen and elastin measurements in skin dermis. Changes of dermal collagen and elastin content are characteristic for skin aging as well as for pathological skin conditions.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
I. Riemann, M. Schwarz, F. Stracke, A. Ehlers, E. Dimitrow, M. Kaatz, K. König, R. Le Harzic, "New developments in two-photon analysis of human skin in vivo", Proc. SPIE 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX, 720306 (13 February 2009); doi: 10.1117/12.808770; https://doi.org/10.1117/12.808770
PROCEEDINGS
9 PAGES


SHARE
RELATED CONTENT

First multiphoton tomography of brain in man
Proceedings of SPIE (March 09 2016)
Multiphoton tomography of the human eye
Proceedings of SPIE (February 21 2017)
In vivo multiphoton tomography of skin cancer
Proceedings of SPIE (February 23 2006)
Current developments in clinical multiphoton tomography
Proceedings of SPIE (February 26 2010)

Back to Top