Coupling confocal fluorescence microscopy and microfluidic device for single molecule detection

Guoqing Shen; Baohe Chang; Bryan D. Dickerson; Xiaoxuan Li; Lloyd M. Davis

doi:10.1117/12.807962

23 February 2009 Coupling confocal fluorescence microscopy and microfluidic device for single molecule detection

Guoqing Shen, Baohe Chang, Bryan D. Dickerson, Xiaoxuan Li, Lloyd M. Davis

Proceedings Volume 7207, Microfluidics, BioMEMS, and Medical Microsystems VII; 72070H (2009) https://doi.org/10.1117/12.807962
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

The ability to accurately measure the mobility of particles at low concentrations in small volumes is very useful for a broad range of applications. The coupling of micro- and nano-fluidic devices and confocal microscopy offers an efficient and rapid technique for multiplexed single molecule detection and analysis. Microfluidic channels at micron and sub-micron scales were designed and fabricated on fused silica wafers. Fluorescence correlation spectroscopy and fluorescence lifetime were applied to measure and analyze the mobility of fluorescent species in micro-droplets, micro-channels, and nano-channels. The experimental results show

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Guoqing Shen, Baohe Chang, Bryan D. Dickerson, Xiaoxuan Li, and Lloyd M. Davis "Coupling confocal fluorescence microscopy and microfluidic device for single molecule detection", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070H (23 February 2009); https://doi.org/10.1117/12.807962

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