3 February 2009 Quantitative phase-contrast digital holographic microscopy for cell dynamic evaluation
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Abstract
The laser microbeam uses lasers to alter and/or to ablate intracellular organelles and cellular and tissue samples, and, today, has become an important tool for cell biologists to study the molecular mechanism of complex biological systems by removing individual cells or sub-cellular organelles. However, absolute quantitation of the localized alteration/damage to transparent phase objects, such as the cell membrane or chromosomes, was not possible using conventional phase-contrast or differential interference contrast microscopy. We report the development of phase-contrast digital holographic microscopy for quantitative evaluation of cell dynamic changes in real time during laser microsurgery. Quantitative phase images are recorded during the process of laser microsurgery and thus, the dynamic change in phase can be continuously evaluated. Out-of-focus organelles are re-focused by numerical reconstruction algorithms.
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Lingfeng Yu, Lingfeng Yu, Samarendra Mohanty, Samarendra Mohanty, Michael W. Berns, Michael W. Berns, Zhongping Chen, Zhongping Chen, } "Quantitative phase-contrast digital holographic microscopy for cell dynamic evaluation", Proc. SPIE 7233, Practical Holography XXIII: Materials and Applications, 72330C (3 February 2009); doi: 10.1117/12.808038; https://doi.org/10.1117/12.808038
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