Molecular diagnostic applications for pathogen detections require the ability to separate pathogens such as
bacteria, viruses, etc., from a biological sample of blood or saliva. Over the past several years,
conventional two-dimensional active microarrays have been used with success for the manipulation of
biomolecules including DNA. However, they have a major drawback of inability to process relatively 'largevolume'
samples useful in infectious disease diagnostics applications. This paper presents an active
microarray of three-dimensional carbon electrodes that exploits electrokinetic forces for transport,
accumulation, and hybridization of charged bio-molecules with an added advantage of large volume
capability. Tall 3-dimensional carbon microelectrode posts are fabricated using C-MEMS (Carbon MEMS)
technology that is emerging as a very exciting research area since carbon has fascinating physical,
chemical, mechanical and electrical properties in addition to its low cost. The chip fabricated using CMEMS
technology is packaged and its efficiency of separation and accumulation of charged particle
established by manipulating negatively charged polycarboxylate 2 μm beads in 50 mM histidine buffer.