30 December 2008 The analysis of surface treatment of PDMS on prostate cancer and smooth muscle cells
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The analysis of cellular activity when exposed to polydimethylsiloxane (PDMS) is necessary as this material has been used in various applications such as tissue engineering and microfluidic devices for cellular studies due to the polymer's unique mechanical properties. In this particular study, we investigated the effects of corona surface treated PDMS with different cross-linker ratios on cellular activities by analyzing prostate cancer cell (PC-3) and vascular smooth muscle cell (VSMC) adhesion and proliferation. Both cell lines were subjected to a thin PDMS layer immediately after and 24 hours after corona treatment. The results indicated steady cell adhesion and proliferation rates for both smooth muscle and prostate cancer cells when seeded onto PDMS 24 hours after corona surface treatment, but significantly less cell adhesion when seeded immediately after activation and controls (PDMS without any treatment). These results would allow future PC-3 and VSMC experiments to be performed in a PDMS environment that is not detrimental for adhesion and proliferation.
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Cory Huggins, Cory Huggins, Smitha M. N. Rao, Smitha M. N. Rao, Kytai Nguyen, Kytai Nguyen, J.-C. Chiao, J.-C. Chiao, } "The analysis of surface treatment of PDMS on prostate cancer and smooth muscle cells", Proc. SPIE 7269, Micro- and Nanotechnology: Materials, Processes, Packaging, and Systems IV, 726914 (30 December 2008); doi: 10.1117/12.810763; https://doi.org/10.1117/12.810763

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