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30 December 2008 Magnetic DNA separation process with functionalized magnetic silica nanoparticles
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Abstract
Work presented here describes a simple and convenient process to highly efficient and direct DNA separation with functionalized silica-coated magnetic nanoparticles. Iron oxide magnetic nanoparticles and silica-coated magnetic nanoparticles were obtained uniformly and the silica coating thickness could be easily controlled in a range from 10 to 50 nm by changing the concentration of silica precursor (TEOS) including the controlled magnetic strength and particle size. A change in the surface hydrophilicity on the nanoparticles was introduced by aminosilanization to enhance the selective DNA separation resulting from electrostatic interaction. The efficiency of the DNA separation was explored via the function of the amino-group numbers, particle size, the amount of the nanoparticles used, and the concentration of NaCl salt.
© (2008) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jiho Lee and Jeong Ho Chang "Magnetic DNA separation process with functionalized magnetic silica nanoparticles", Proc. SPIE 7270, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 72701C (30 December 2008); https://doi.org/10.1117/12.814120
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