6 March 2009 Crushing cells in microfluidic channels and observing with optical imaging
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Abstract
A cell lysis device is developed. It is designed for miniaturing bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of multi-electrode pairs to apply electric fields to cells. The method of electric field lysing is adopted because it can greatly simplify purifying steps for preparation of biological samples compared with conventional chemical methods. BHK21 cells are tested with the device and the lysis of BHK21 is observed with a microscope. The experimental observation suggests that BHK21 cells are lysed by the pulsed electric field. It takes inverted microscope as the main light path system, collects pictures with 3 Mega Pixel CCD camera and deals them with processing software of micro-digital image. The experiment shows that this optical testing system could automatically identify and count BHK21 in micro-windows. For crushing BHK21 cells, the most suitable voltage range and pulse width are 3V and 3ms.
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Jianjun Chen, Chenggang Chai, Jun Wang, Honghua Liao, Heyou Han, Jun Yu, "Crushing cells in microfluidic channels and observing with optical imaging", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800E (6 March 2009); doi: 10.1117/12.823300; https://doi.org/10.1117/12.823300
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