6 March 2009 The repair of low dose UV light-induced damage to human skin DNA in condition of trace amount Mg 2+
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Abstract
Ultraviolet light-induced damage to human skin DNA was widely investigated. The primary mechanism of this damage contributed to form cyclobutane pyrimidine dimmers (CPDs). Although the distribution of UV light-induced CPDs within a defined sequence is similar, the damage in cellular environment which shields the nuclear DNA was higher than that in organism in apparent dose. So we use low UVB light as main study agent. Low dose UV-irradiated HDF-a cells (Human Dermal Fibroblasts-adult cells) which is weaker than epidermic cells were cultured with DMEM at different trace amount of Mg2+ (0mmol/L , 0.1mmol/L , 0.2mmol/L, 0.4mmol/L, 0.8mmol/L, 1.2mmol/L) free-serum DMEM and the repair of DNA strands injured were observed. Treat these cells with DNA strand breaks detection, photoproducts detection and the repair of photoproducts detection. Then quantitate the role of trace amount Mg2+ in repair of UV light-induced damage to human skin. The experiment results indicated that epidermic cells have capability of resistance to UV-radiation at a certain extent. And Mg2+ can regulate the UV-induced damage repair and relative vitality. It can offer a rationale and experiment data to relieve UV light-induced skin disease.
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Fang Gao, Fang Gao, Zhouyi Guo, Zhouyi Guo, Changchun Zheng, Changchun Zheng, Rui Wang, Rui Wang, Zhiming Liu, Zhiming Liu, Pei Meng, Pei Meng, Juan Zhai, Juan Zhai, } "The repair of low dose UV light-induced damage to human skin DNA in condition of trace amount Mg 2+", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800L (6 March 2009); doi: 10.1117/12.821458; https://doi.org/10.1117/12.821458
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