Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging

Suihua Ma; Le Liu; Weiping Lu; Yaou Zhang; Yonghong He; Jihua Guo

doi:10.1117/12.823908

6 March 2009 Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging

Suihua Ma, Le Liu, Weiping Lu, Yaou Zhang, Yonghong He, Jihua Guo

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72801H (2009) https://doi.org/10.1117/12.823908
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

SPR sensors have become a high sensitive and label free method for characterizing and quantifying chemical and biochemical interactions. However, the relations between the SPR refractive index response and the property (such as concentrations) of biochemical probes are still lacking. In this paper, an experimental study on the SPR responses of varies concentrations of Legionella pneumophila mip DNA probes is presented. We developed a novel two-dimensional SPR sensing technique-parallel scan spectral SPR imaging-to detect an array of mip gene probes. This technique offers quantitative refractive index information with a high sensing throughput. By detecting mip DNA probes with different concentrations, we obtained the relations between the SPR refractive index response and the concentrations of mip DNA probes. These results are valuable for design and developing SPR based mip gene biochips.

Citation Download Citation

Suihua Ma, Le Liu, Weiping Lu, Yaou Zhang, Yonghong He, and Jihua Guo "Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801H (6 March 2009); https://doi.org/10.1117/12.823908

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