6 March 2009 Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging
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Abstract
SPR sensors have become a high sensitive and label free method for characterizing and quantifying chemical and biochemical interactions. However, the relations between the SPR refractive index response and the property (such as concentrations) of biochemical probes are still lacking. In this paper, an experimental study on the SPR responses of varies concentrations of Legionella pneumophila mip DNA probes is presented. We developed a novel two-dimensional SPR sensing technique-parallel scan spectral SPR imaging-to detect an array of mip gene probes. This technique offers quantitative refractive index information with a high sensing throughput. By detecting mip DNA probes with different concentrations, we obtained the relations between the SPR refractive index response and the concentrations of mip DNA probes. These results are valuable for design and developing SPR based mip gene biochips.
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Suihua Ma, Suihua Ma, Le Liu, Le Liu, Weiping Lu, Weiping Lu, Yaou Zhang, Yaou Zhang, Yonghong He, Yonghong He, Jihua Guo, Jihua Guo, } "Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801H (6 March 2009); doi: 10.1117/12.823908; https://doi.org/10.1117/12.823908
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