Effect of the prosurvival protein MCL-1 on photodynamic therapy induced apoptosis

Jiaxing Song; Yunlong Li; Yanchun Wei

doi:10.1117/12.822047

6 March 2009 Effect of the prosurvival protein MCL-1 on photodynamic therapy induced apoptosis

Jiaxing Song, Yunlong Li, Yanchun Wei

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72801T (2009) https://doi.org/10.1117/12.822047
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

Photodynamic Therapy (PDT), a promising and approved therapeutics for neoplastic and vascular disease, could induce expression of angiogenic and survival molecules including vascular endothelial growth factor, cyclooxygenase-2 (COX-2), and MCL-1. However, the precise effect of MCL-1 which is a member of the bcl-2 family on PDT is not well characterized. The work described here was aimed at determining whether expression of MCL-1 could exhibit a side effect on PDT. The results showed that expression of MCL-1 was increased prominently after Hela cells treated with PDT. By using confocal fluorescence microscopy and cell viability assay, it was found that overexpression of MCL-1 could have a vital influence on Photofrin-PDT-mediated apoptosis. Moreover, further analyses indicated that MCL-1 delayed translocation of Bax and release of cytochrome c from mitochrondria, further leading to cause a delay in cell apoptosis in Hela cells treated with PDT. The process was associated with MCL-1 interaction directly with Bax. In conclusion, overexpression of MCL-1 plays an important part in efficiency of PDT killing tumor cells, in other words, MCL-1 overexpression decreases PDT induced apoptosis through directly interacting with Bax. These results suggest that targeting MCL-1 expression might be a highly effective therapeutic measure for promoting the tumoricidal effectiveness of PDT.

Citation Download Citation

Jiaxing Song, Yunlong Li, and Yanchun Wei "Effect of the prosurvival protein MCL-1 on photodynamic therapy induced apoptosis", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801T (6 March 2009); https://doi.org/10.1117/12.822047

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available