Five-color fluorescent imaging in living tumor cells

Liang Wang; Jie Yang; Jun Chu; Qingming Luo; Zhihong Zhang

doi:10.1117/12.824017

6 March 2009 Five-color fluorescent imaging in living tumor cells

Liang Wang, Jie Yang, Jun Chu, Qingming Luo, Zhihong Zhang

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72801Z (2009) https://doi.org/10.1117/12.824017
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

The fluorescent probes based on fluorescent proteins (FP) have been widely used to investigate the molecules of interest in living cells. It is well-known that the molecular events in the living cells are very complicate and all of the cell activities are involved by multi-molecular interaction. With the development of novel fluorescent protein mutants and imaging technology, the molecular signal in living cells could be detected accurately. In this study, with the appropriate targeting signals, the fluorescent proteins were localized to plasma membrane (Rac1-mCerulean), Golgi membrane (EYFP-go), ER membrane (RFP2-er), mitochondrial membrane (RFP1-mt). Cultured Hela cells were cotransfected with these four plasmids, and 36 h later, labeled with Hoechst33258 which located in the nucleus of a living cell. Using a confocal microscopy, with 405 nm, 458 nm and 514 nm laser lines employed respectively, a five-color fluorescent image was obtained in which five subcellular structures were clearly shown in living cells. The technique of multi-color imaging in a single cell provides a powerful tool to simultaneously study the multi-molecular events in living cells.

Citation Download Citation

Liang Wang, Jie Yang, Jun Chu, Qingming Luo, and Zhihong Zhang "Five-color fluorescent imaging in living tumor cells", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72801Z (6 March 2009); https://doi.org/10.1117/12.824017

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