6 March 2009 Using low-frequency ultrasound to improve the optical clearing of porcine skin
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The glycerol used as an enhancer for tissue optical clearing technique has been researched. However, using it and a physical way of ultrasound enhance optical clearing of tissue reported a few. We researched that the ultrasound whether can improve the optical clearing of dealt with 80% glycerol tissue. The fresh porcine skins divided into four groups. The first group was not dealt with by ultrasound and 80% glycerol, the second group was dealt with by only ultrasound, the third group was dealt with by 80% glycerol and no by ultrasound, and the fourth group was dealt with by both 80% glycerol and ultrasound. And we measured changes in optical scattering of the porcine skins under treatment with OCT. From the OCT images show that the fourth group changed very faster than the other's during the 0~15 min. And it can be clearly seen that there is a significant improvement in the light penetration depth and imaging contrast in a shorter time. It is possible that the low-frequency ultrasound can make disordering of the stratum corneum lipids of the porcine skin (because the cavitation has happened), and improve the speed of 80% glycerol through the stratum corneum of skin. These results proved that using 80% glycerol with the ultrasound can better improve the optical clearing of tissue.
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Huiqing Zhong, Huiqing Zhong, Zhouyi Guo, Zhouyi Guo, Huajiang Wei, Huajiang Wei, Zude Zhang, Zude Zhang, Changchun Zeng, Changchun Zeng, Juan Zhai, Juan Zhai, Yonghong He, Yonghong He, "Using low-frequency ultrasound to improve the optical clearing of porcine skin", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72802N (6 March 2009); doi: 10.1117/12.821337; https://doi.org/10.1117/12.821337


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Proceedings of SPIE (September 11 2002)

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