30 April 2009 System for rapid detection of antibiotic resistance of airborne pathogens
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Abstract
This project uses function-based detection via a fundamental understanding of the genetic markers of AR to distinguish harmful organisms from innocuous ones. This approach circumvents complex analyses to unravel the taxonomic details of 1399 pathogen species, enormously simplifying detection requirements. Laval Hospital's fast permeabilization strategy enables AR revelation in <1hr. Packaging the AR protocols in liquid-processing cartridges and coupling these to our in-house miniature fiber optic flow cell (FOFC) provides first responders with timely information on-site. INO's FOFC platform consists of a specialty optical fiber through which a hole is transversally bored by laser micromachining. The analyte solution is injected into the hole of the fiber and the particles are detected and counted. The advantage with respect to classic free space FC is that alignment occurs in the fabrication process only and complex excitation and collection optics are replaced by optical fibers. Moreover, we use a sheathless configuration which has the advantage of increase the portability of the system, to reduce excess biohazard material and the need for weekly maintenance. In this paper we present the principle of our FOFC along with a, demonstration of the basic capability of the platform for detection of bacillus cereus spores using permeabilized staining.
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M. Fortin, M. Fortin, I. Noiseux, I. Noiseux, L. Mouslinkina, L. Mouslinkina, M. L. Vernon, M. L. Vernon, C. Laflamme, C. Laflamme, G. Filion, G. Filion, C. Duchaine, C. Duchaine, J. Ho, J. Ho, } "System for rapid detection of antibiotic resistance of airborne pathogens", Proc. SPIE 7312, Advanced Environmental, Chemical, and Biological Sensing Technologies VI, 731203 (30 April 2009); doi: 10.1117/12.818783; https://doi.org/10.1117/12.818783
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