11 May 2009 Antibody-functionalized magnetic nanowires for cell purification
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Rapid and efficient cell purification remains challenging. The use of ferromagnetic Ni nanowires for cell purification is considered superior over magnetic beads. In this study, we explored the opportunity to improve cell purification by using antibody-functionalized Ni nanowires. Antibody (anti-CD31) against mouse endothelial cells (MS-1) was conjugated to Ni nanowire surface by self-assembled monolayers (SAMs) and chemical covalent reaction. The antibody functionalized nanowires were used to purify the MS-1 from a mixture of MS-1 and mouse fibroblast cells (3T3). The nanowire-bound cells were magnetically separated to determine the separation yield of target cells. Furthermore, the proliferation of nanowire-bound cells was studied by MTT cell proliferation assay. This work demonstrates that antibody-functionalized Ni nanowires provide an effective mean to separate cells.
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Ning Gao, Ning Gao, Xiaochuan Yang, Xiaochuan Yang, Yao-Tsan Tsai, Yao-Tsan Tsai, Geh Meh Chu, Geh Meh Chu, Hongjun Wang, Hongjun Wang, Eui-Hyeok Yang, Eui-Hyeok Yang, "Antibody-functionalized magnetic nanowires for cell purification", Proc. SPIE 7318, Micro- and Nanotechnology Sensors, Systems, and Applications, 73181E (11 May 2009); doi: 10.1117/12.818402; https://doi.org/10.1117/12.818402

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