6 May 2009 Portable Raman instrument for rapid biological agent detection and identification
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Abstract
The rapid and sensitive identification of biological species is a critical need for the 1st responder and military communities. Raman spectroscopy is a powerful tool for substance identification that has gained popularity with the respective communities due to the increasing availability of portable Raman spectrometers. Attempts to use Raman spectroscopy for the direct identification of biological pathogens has been hindered by the complexity of the generated Raman spectrum. We report here the use of a sandwich immunoassay containing antibody modified magnetic beads to capture and concentrate target analytes in solution and Surface Enhanced Raman Spectroscopy (SERS) tags conjugated with these same antibodies for specific detection. Using this approach, the biological complexity of a microorganism can be translated into chemical simplicity and Raman can be used for the identification of biological pathogens. The developed assay has a low limit of detection due to the SERS effect, robust to commonly found white powders interferants, and stable at room temperature over extended period of time. This assay is being implemented into a user-friendly interface to be used in conjunction with the GE Homeland Protection StreetLab MobileTM Raman instrument for rapid, field deployable chemical and biological identification.
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Marie L. Lesaicherre, Marie L. Lesaicherre, Tracy L. Paxon, Tracy L. Paxon, Frank J. Mondello, Frank J. Mondello, Michael C. Burrell, Michael C. Burrell, Amy Linsebigler, Amy Linsebigler, } "Portable Raman instrument for rapid biological agent detection and identification", Proc. SPIE 7319, Next-Generation Spectroscopic Technologies II, 73190C (6 May 2009); doi: 10.1117/12.817799; https://doi.org/10.1117/12.817799
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