29 April 2009 Label-free DNA methylation analysis using the optofluidic ring resonator sensor
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Abstract
We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for the purpose of analyzing the degree of methylation in sample oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, is prone to abnormal behavior that may lead to uncontrolled suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool to explore and monitor the degree of methylation in DNA. Two different approaches are explored, using either bisulfite modification or immunoprecipitation. The methods are compared and the signal response for both methods is characterized.
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Jonathan D. Suter, Daniel J. Howard, Charles W. Caldwell, Huidong Shi, Xudong Fan, "Label-free DNA methylation analysis using the optofluidic ring resonator sensor", Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 732208 (29 April 2009); doi: 10.1117/12.818562; https://doi.org/10.1117/12.818562
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