Simulation modelling for the analysis and the optimal design of SPAD detectors for time-resolved fluorescence measurements

Marina Repich; David Stoppa; Lucio Pancheri; Gian-Franco Dalla Betta

doi:10.1117/12.820430

18 May 2009 Simulation modelling for the analysis and the optimal design of SPAD detectors for time-resolved fluorescence measurements

Marina Repich, David Stoppa, Lucio Pancheri, Gian-Franco Dalla Betta

Author Affiliations +

Proceedings Volume 7355, Photon Counting Applications, Quantum Optics, and Quantum Information Transfer and Processing II; 73550O (2009) https://doi.org/10.1117/12.820430
Event: SPIE Optics + Optoelectronics, 2009, Prague, Czech Republic

Abstract

This paper describes a simulation model (implemented in MATLAB) of a typical setup used for time-resolved fluorescence measurements, including: a laser source, basic fluorescence sample, optics, single-photon avalanche diode and read-out electronics. The correctness of the model has been verified by setting up a simple time-resolved fluorescence measurement using a CMOS SPAD-based detector. The solution of fluorophore (CdSe/ZnS quantum dots in toluene) in a glass capillary was placed above the detecting surface and excited by laser pulses. We have used a time-gating technique with 10-ns observation window shifted at 60-ps time steps across the appropriate time interval. The observed curve corresponds to the convolution of the fluorescence emission and the 10-ns observation window. Simulation accuracy has been verified by comparing the experimental fluorescence decay with the simulated one using chi-square test. The proposed model allows researchers to simulate the behaviour of SPAD detectors with a good accuracy and demonstrates how imperfections in the experimental system can affect the result. The model enables the design of SPAD-based detectors with the best performance for a specific application area.

Citation Download Citation

Marina Repich, David Stoppa, Lucio Pancheri, and Gian-Franco Dalla Betta "Simulation modelling for the analysis and the optimal design of SPAD detectors for time-resolved fluorescence measurements", Proc. SPIE 7355, Photon Counting Applications, Quantum Optics, and Quantum Information Transfer and Processing II, 73550O (18 May 2009); https://doi.org/10.1117/12.820430

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