18 May 2009 Fast detection of air contaminants using immunobiological methods
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The fast and direct identification of possibly pathogenic microorganisms in air is gaining increasing interest due to their threat for public health, e.g. in clinical environments or in clean rooms of food or pharmaceutical industries. We present a new detection method allowing the direct recognition of relevant germs or bacteria via fluorescence-labeled antibodies within less than one hour. In detail, an air-sampling unit passes particles in the relevant size range to a substrate which contains antibodies with fluorescence labels for the detection of a specific microorganism. After the removal of the excess antibodies the optical detection unit comprising reflected-light and epifluorescence microscopy can identify the microorganisms by fast image processing on a single-particle level. First measurements with the system to identify various test particles as well as interfering influences have been performed, in particular with respect to autofluorescence of dust particles. Specific antibodies for the detection of Aspergillus fumigatus spores have been established. The biological test system consists of protein A-coated polymer particles which are detected by a fluorescence-labeled IgG. Furthermore the influence of interfering particles such as dust or debris is discussed.
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Katrin Schmitt, Carsten Bolwien, Gerd Sulz, Wolfgang Koch, Wilhelm Dunkhorst, Hubert Lödding, Katharina Schwarz, Andreas Holländer, Torsten Klockenbring, Stefan Barth, Björn Seidel, Wolfgang Hofbauer, Torsten Rennebarth, and Anna Renzl "Fast detection of air contaminants using immunobiological methods", Proc. SPIE 7362, Smart Sensors, Actuators, and MEMS IV, 736207 (18 May 2009); doi: 10.1117/12.822672; https://doi.org/10.1117/12.822672

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