3.0-3.7&mu;m infrared sensor system for cell analysis

Sander van den Driesche; Wojciech Witarski; Michael J. Vellekoop

doi:10.1117/12.822058

18 May 2009 3.0-3.7μm infrared sensor system for cell analysis

Sander van den Driesche, Wojciech Witarski, Michael J. Vellekoop

Proceedings Volume 7362, Smart Sensors, Actuators, and MEMS IV; 73620Y (2009) https://doi.org/10.1117/12.822058
Event: SPIE Europe Microtechnologies for the New Millennium, 2009, Dresden, Germany

Abstract

In this contribution we present a novel LED-photodiode based infrared absorbance sensor in the wavelength range of 3.0 - 3.7 μm for cell analysis. Instead of using time consuming and expensive labelling and staining techniques to distinguish healthy from malignant cell types, this IR sensor system can perform faster, cheaper and without the need of additional chemicals. Depending on the used narrow bandpass filters, absorbance due to specific molecular vibration can be measured, such as the functional absorbance peaks at 3.38 μm (CH₃-antisymmetric stretch), 3.42 μm (CH₂- antisymmetric stretch), 3.48 μm (CH₃-symmetric stretch) and 3.51 μm (CH₂-symmetric stretch). For normalization and baseline correction the absorbance at wavelengths 3.33 and 3.57 μm are used. By recording the IR absorbance spectra of healthy and malignant epithelial kidney cell lines with an IR spectroscope, we found significant differences in the absorbance ratio 3.51 μm / 3.42 μm (CH₂-symmetric/antisymmetric stretch). This result has led us to a sensor concept where only four wavelengths are being measured. In the 3.0 - 3.7 μm wavelength region a low cost LED-photodiode system can be used instead of a spectroscope. Yeast cells, which also contain the CH₂ symmetric and antisymmetric stretch bands, are used to validate this sensor system and to make a first comparison of the system to spectroscopic recordings. Sensor experiments on dried spots of baker's yeast on calcium-fluoride slides yielded a comparable CH₂ stretch ratio with the IR spectroscope measurement. This confirms the usability of the sensor to measure the CH₂ stretch ratio and its potential for fast, label-free and low cost screening of cell samples.

Citation Download Citation

Sander van den Driesche, Wojciech Witarski, and Michael J. Vellekoop "3.0-3.7μm infrared sensor system for cell analysis", Proc. SPIE 7362, Smart Sensors, Actuators, and MEMS IV, 73620Y (18 May 2009); https://doi.org/10.1117/12.822058

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