26 May 2009 Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)
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Abstract
The detection of specific DNA sequences for the analysis of mutations as well as the detection of proteins gains increasing importance in the field of point-of-care diagnostics. Here, a novel low-cost lab-on-a-chip system for label-free detection of DNA hybridization and protein-protein interaction is introduced. The platform consists of a reader with disposable SPR chips produced by injection moulding. Micro optical elements are integrated into the chip to accomplish a simple connection to the optical reader. Automated, software-controlled reagent handling is achieved by a temperaturecontrolled microfluidic system comprising a syringe pump and a switching valve. The sensing area can be separated into maximum 40 parts for parallel analysis. Patterned functionalization is achieved by inverse micro contact printing. Several application examples, ranging from on-chip DNA hybridization up to the detection of antibodies inside diluted human blood serum, will be demonstrated.
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F. Sonntag, S. Schmieder, N. Danz, M. Mertig, N. Schilling, U. Klotzbach, and E. Beyer "Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR)", Proc. SPIE 7365, Bioengineered and Bioinspired Systems IV, 73650Q (26 May 2009); doi: 10.1117/12.821467; https://doi.org/10.1117/12.821467
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