20 May 2009 Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy
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Abstract
We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.
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Silvia Soria, Silvia Soria, Franco Quercioli, Franco Quercioli, Raffaella Mercatelli, Raffaella Mercatelli, Federica Bianco, Federica Bianco, Ilaria Cacciari, Ilaria Cacciari, Stefano Pelli, Stefano Pelli, Giancarlo Righini, Giancarlo Righini, } "Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy", Proc. SPIE 7366, Photonic Materials, Devices, and Applications III, 73660R (20 May 2009); doi: 10.1117/12.821199; https://doi.org/10.1117/12.821199
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