A novel emerging technique for the label-free analysis of nanoparticles including biomolecules using optical
micro cavity resonance of whispering-gallery-type modes is being developed. Schemes of such a method
based on microsphere melted by laser on the tip of a standard single mode fiber optical cable with a laser and
free microsphere matrix have been developed. Using a calibration principal of ultra high resolution
spectroscopy based on such a scheme the method is being transformed to make further development for
The sensitivity of developed schemes has been tested to refractive index changes by monitoring the
magnitude of the whispering gallery modes spectral shift. Water solutions of ethanol, glucose, vitamin C and
biotin have been used. Some other schemes using similar principals: stand-alone, array and matrix
microsphere resonators, liquid core optical ring resonators are also being under development. The influences
of the gap in whispering-gallery modes on energy coupling, resonance quality and frequency have been
investigated. An optimum gap for sensing applications has been defined at the half maximum energy coupling
where both the Q factor and coupling efficiency are high and the resonance frequency is little affected by the
Developed schemes have been demonstrated to be a promising technology platform for sensitive, lab-on-chip
type sensor which can be used for development of diagnostic tools for different biological molecules, e.g.
proteins, oligonucleotides, oligosaccharides, lipids, small molecules, viral particles, cells as well as in
different experimental contexts e.g. proteomics, genomics, drug discovery, and membrane studies.