Dose limited fluorescence microscopy of living cells

Herbert Schneckenburger; Michael Wagner; Petra Weber; Sarah Schickinger; Thomas Bruns; Wolfgang S. L. Strauss

doi:10.1117/12.831547

1 July 2009 Dose limited fluorescence microscopy of living cells

Herbert Schneckenburger, Michael Wagner, Petra Weber, Sarah Schickinger, Thomas Bruns, Wolfgang S. L. Strauss

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670E (2009) https://doi.org/10.1117/12.831547
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Light dose plays an important role for maintaining viability in optical microscopy of living cells. Therefore, a colony forming assay was established, and non-phototoxic light doses were determined for glioblastoma cells. These doses ranged from a few 1 J/cm² or even less for cells incubated with fluorescence markers or photosensitizers up to about 100 J/cm² for non-incubated cells. Microscopic methods were adapted to those light doses, and often wide field methods appeared to be more appropriate than laser scanning methods.

Citation Download Citation

Herbert Schneckenburger, Michael Wagner, Petra Weber, Sarah Schickinger, Thomas Bruns, and Wolfgang S. L. Strauss "Dose limited fluorescence microscopy of living cells", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670E (1 July 2009); https://doi.org/10.1117/12.831547

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