1 July 2009 Optically sectioned imaging by oblique plane microscopy
Author Affiliations +
Proceedings Volume 7367, Advanced Microscopy Techniques; 73670H (2009); doi: 10.1117/12.831543
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany
An optically sectioning microscopy technique based on oblique selective plane illumination combined with oblique imaging is described. The same high numerical aperture lens is used to both illuminate and image the specimen and correction optics are employed to tilt the focal plane of the imaging system so that the imaged plane aligns with the illuminated plane in the specimen. An optically sectioned image is obtained without the use of moving parts or image processing and this technique therefore has the potential to be used for very high speed optically sectioned microscopy. As only the part of the specimen that is being imaged is illuminated then the photobleaching and phototoxicity of this method is low compared to conventional microscopy techniques.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
C. Dunsby, "Optically sectioned imaging by oblique plane microscopy", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670H (1 July 2009); doi: 10.1117/12.831543; https://doi.org/10.1117/12.831543


New waves in light microscopy
Proceedings of SPIE (August 17 1994)
Knowledge-driven image analysis of cell structures
Proceedings of SPIE (May 01 1991)
Optically sectioned imaging by oblique plane microscopy
Proceedings of SPIE (February 28 2011)

Back to Top