Harmonic holographic microscopy with circularly polarized excitation

Chia-Lung Hsieh; Rachel Grange; Ye Pu; Demetri Psaltis

doi:10.1117/12.831506

1 July 2009 Harmonic holographic microscopy with circularly polarized excitation

Chia-Lung Hsieh, Rachel Grange, Ye Pu, Demetri Psaltis

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670R (2009) https://doi.org/10.1117/12.831506
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We have developed a luminescent marker using the second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and broad flexibility in the choice of excitation wavelength. In this paper, we report the SHG response of BaTiO3 nanocrystals under a circularly polarized excitation. We observed a more uniform SHG signal intensity from nanocrystals of different crystal orientations under a circularly polarized excitation. The threedimensional (3D) SHG fields generated from the SHRIMPs were recorded by a harmonic holographic microscope. Submicron resolution in both lateral and axial directions has been achieved. We show that the circularly polarized excitation is useful for harmonic holographic microscopy.

Citation Download Citation

Chia-Lung Hsieh, Rachel Grange, Ye Pu, and Demetri Psaltis "Harmonic holographic microscopy with circularly polarized excitation", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670R (1 July 2009); https://doi.org/10.1117/12.831506

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