Quasi white light multiphoton imaging

C. de Mauro; D. Alfieri; M. Arrigoni; D. Armstrong; F. S. Pavone

doi:10.1117/12.831549

1 July 2009 Quasi white light multiphoton imaging

C. de Mauro, D. Alfieri, M. Arrigoni, D. Armstrong, F. S. Pavone

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670U (2009) https://doi.org/10.1117/12.831549
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We describe the realization and characterization of a broadband, high power density and fully spectrally controllable source, suitable for multiphoton imaging of biological samples. We used a photonic crystal fiber (PCF) with selected dispersive and non-linear properties, in order to generate, when pumped with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), a smooth continuum in the 700nm-950nm region, with average power density grater than 2mW/nm. Time distribution of the generated spectrum has been measured with autocorrelation technique. Axial and lateral resolution obtained with a scanning multiphoton system has been determined to be near the theoretical limit. The possibility of two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Future developments and different applications are also discussed.

Citation Download Citation

C. de Mauro, D. Alfieri, M. Arrigoni, D. Armstrong, and F. S. Pavone "Quasi white light multiphoton imaging", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670U (1 July 2009); https://doi.org/10.1117/12.831549

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available