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1 July 2009 Quasi white light multiphoton imaging
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Abstract
We describe the realization and characterization of a broadband, high power density and fully spectrally controllable source, suitable for multiphoton imaging of biological samples. We used a photonic crystal fiber (PCF) with selected dispersive and non-linear properties, in order to generate, when pumped with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), a smooth continuum in the 700nm-950nm region, with average power density grater than 2mW/nm. Time distribution of the generated spectrum has been measured with autocorrelation technique. Axial and lateral resolution obtained with a scanning multiphoton system has been determined to be near the theoretical limit. The possibility of two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Future developments and different applications are also discussed.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
C. de Mauro, D. Alfieri, M. Arrigoni, D. Armstrong, and F. S. Pavone "Quasi white light multiphoton imaging", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670U (1 July 2009); https://doi.org/10.1117/12.831549
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