High-resolution fluorescence microscopy using three-dimensional structured illumination

P. F. Gardeazabal Rodriguez; P. Blandin; I. Maksimovic; E, Sepulveda; E. Muro; B. Dubertret; V. Loriette

doi:10.1117/12.831477

1 July 2009 High-resolution fluorescence microscopy using three-dimensional structured illumination

P. F. Gardeazabal Rodriguez, P. Blandin, I. Maksimovic, E, Sepulveda, E. Muro, B. Dubertret, V. Loriette

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670X (2009) https://doi.org/10.1117/12.831477
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We developed a high-resolution microscope based on three-dimensional structured illumination generated with two spatial light modulators. This setup enables both lateral resolution improvement by a factor two and axial localization of point like objects with nanometric precision.

Citation Download Citation

P. F. Gardeazabal Rodriguez, P. Blandin, I. Maksimovic, E, Sepulveda, E. Muro, B. Dubertret, and V. Loriette "High-resolution fluorescence microscopy using three-dimensional structured illumination", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670X (1 July 2009); https://doi.org/10.1117/12.831477

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