1 July 2009 FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope
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Abstract
We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.
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V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M. C. Potier, F. Druon, P. Georges, S. Lévêque-Fort, "FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope", Proc. SPIE 7367, Advanced Microscopy Techniques, 736711 (1 July 2009); doi: 10.1117/12.831537; https://doi.org/10.1117/12.831537
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